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Steps in A Typical Knockout Procedure

  • BAC library screening (3-4 weeks)
  • Knockout vector construction (8 weeks)
  • Transfection, drug selection, picking, expanding, freezing (6 weeks)
  • Screening for positive ES clones (3 weeks)
  • Expanding positive ES clones (3 weeks)
  • Reconfirmation (2 weeks)
  • Karyotyping positive ES clones (2 weeks)
  • Blastocyst inject (1 week)
  • Breeding for germline transmission (1-6 months)
  • Breeding for homozygosity (2-3 months)
  • service inquiry/request
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    Adenovirus Service from Vector Biolabs