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Steps
in A Typical Knockout Procedure
BAC library screening (3-4 weeks)
Knockout vector construction (8 weeks)
Transfection, drug selection, picking, expanding, freezing
(6 weeks)
Screening for positive ES clones (3 weeks)
Expanding positive ES clones (3 weeks)
Reconfirmation (2 weeks)
Karyotyping positive ES clones (2 weeks)
Blastocyst inject (1 week)
Breeding for germline transmission (1-6 months)
Breeding for homozygosity (2-3 months)
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inquiry/request
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